Oxygen-independent glucose microsensor based on glucose oxidase |
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Authors: | Carel Cronenberg Bert van Groen Dirk de Beer Han van den Heuvel |
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Affiliation: | Department of Chemical Engineering and Biotechnological Centre, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam The Netherlands |
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Abstract: | A glucose oxidase-based needle-type microsensor is described that is independent of the local oxygen concentration. The sensor consists of an enzyme-coated platinum microelectrode that is inserted in a glass capillary with a tip of 5–25 μm, and connected to the environment via an agar membrane. Oxygen is supplied to the enzyme coating from the shaft of the capillary. Depending on the electrode configuration, the sensor has a response time of 1–10 s, and the measuring range extends from 0.01 to 0.4 mmol l?1 or from 0.4 to 10 mmol l?1. The signal is not affected by stirring. |
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Keywords: | Amperometry Glucose Enzyme electrodes |
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