葡萄糖氧化酶光敏氧化反应过程的毛细管电泳法监测

生物学研究表明，叶琳和酞著衍生物对癌变组织具有亲和性1]．此类物质在吸收特定波长的光之后产生活性氧，可光敏氧化生物分子，并杀死癌细胞．光动力疗法对脑癌、颈癌和服癌等浅层癌具有明显的疗效2]，研究各种光敏剂与生物大分子的作用机理3，4]，对筛选新型光疗药物，改进光疗效果具有重要价值．癌细胞中葡萄糖的代谢非常旺盛，若阻断这种代谢，有可能实现对癌细胞进行代谢调控的目的．因此，本文选用葡萄糖氧化酶为模型化合物，用毛细管电泳法监测了4种新型光敏剂ZnPcS4，ZnPc（NHCH2COOH）4，C1A1PcS，Por－Phyrine－like]…

Studies on the interaction of Photosensitizers and Glucose Oxidase Using Capillary Electrophoresis

The interaction of glucose oxidase (GOD) and four photosensitizers, ZnpcS4,ZnPc(NHCH2COOH)4, CIAIPcS, and porphyrine-like, were monitored by capillaryelectrophoresis. GOD, its degenerated products and photosensitizers were simultaneouslyseparated in a buffer of 300 mmol/L borate at PH 8.5 on a commercial CE instrument. Bycomparison of the electropherograms of GOD as well as the mixture of GOD and photosensitizerswithout/with irradiation of different time, it was found that photosensitized oxidationsbetween GOD and different photosensitizers have different reaction mechanisms and kinetics.