Patterning of 293T fibroblasts on a mica surface |
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Authors: | Sen Hou Xin-Xin Li Xiao-Yu Li Xi-Zeng Feng Li Guan Yan-Lian Yang Chen Wang |
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Institution: | (1) College of Life Science, Nankai University, Tianjin, 300071, China;(2) National Center for Nanoscience and Technology, Beijing, 100080, China |
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Abstract: | Controllable cell growth on the defined areas of surfaces is important for potential applications in biosensor fabrication
and tissue engineering. In this study, controllable cell growth was achieved by culturing 293 T fibroblast cells on a mica
surface which had been patterned with collagen strips by a microcontact printing (μCP) technique. The collagen area was designed
to support cell adhesion and the native mica surface was designed to repel cell adhesion. Consequently, the resulting cell
patterns should follow the micro-patterns of the collagen. X-ray photoelectron spectroscopy (XPS), water contact angle (WCA)
measurement, atomic-force microscope (AFM) observation, and force-curve measurement were used to monitor property changes
before and after the collagen adsorption process. Further data showed that the patterned cells were of good viability and
able to perform a gene-transfection experiment in vitro. This technique should be of potential applications in the fields
of biosensor fabrication and tissue engineering.
Figure Controllable cells growth has been achieved by culturing 293T fibroblast cells on the mica surface which had been patterned
with collagen strips by microcontact printing (μCP) technique |
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Keywords: | Cell adhesion Microcontact printing Collagen Gene transfection Mica |
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