Detection of the illegal use of ethinylestradiol in cattle urine by gas chromatography-mass spectrometry |
| |
| Authors: | E Daeseleire A De Guesquière C Van Peteghem |
| |
| Affiliation: | Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium. |
| |
| Abstract: | A method for the detection of ethinylestradiol in cattle urine is described, based on enzymic hydrolysis of the sample, clean-up by means of disposable octadecyl and amino solid-phase extraction columns, fractionation by reversed-phase high-performance liquid chromatography, and detection by gas chromatography-mass spectrometry (selected-ion monitoring). Identification is based on both gas chromatographic and mass spectrometric data. The method has been tested on urine samples for a collaborative study and all the results found were correct. |
| |
| Keywords: | |
|
|
