| 应用高效液相色谱和电喷雾质谱联用技术分析鉴定车前草中的苯乙醇苷化合物 |
| |
| 引用本文： | 李丽,刘春明,刘志强,Rong Tsao,刘淑莹.应用高效液相色谱和电喷雾质谱联用技术分析鉴定车前草中的苯乙醇苷化合物[J].中国化学,2009,27(3):541-544. |
| |
| 作者姓名： | 李丽 刘春明 刘志强 Rong Tsao 刘淑莹 |
| |
| 作者单位： | 1 长春应用化学研究所质谱中心，长春 130022 ;2 长春师范学院中心实验室, 长春130032 ;3 Food Research Program, Agriculture & Agri-Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada ; |
| |
| 摘 要： | 本文采用高效液相色谱与电喷雾质谱联用技术在线分析鉴定了车前草提取物中的三种苯乙醇苷化合物。实验采用反相C18色谱柱，0.2%的醋酸水溶液和乙腈梯度洗脱，车前草中的苯乙醇苷化合物得到很好的分离。在电喷雾质谱负离子条件下，获得了三种苯乙醇苷化合物的分子离子峰，分子量信息，进一步通过质谱的源内CID技术得到相应化合物的结构信息。通过得到的这些信息与文献中的已知化合物或标准品对照从而推断出化合物的结构。
|
| 关 键 词： | 车前草 苯乙醇苷化合物 高效液相色谱-电喷雾质谱 |
| 收稿时间： | 2008-6-6 |
| 修稿时间： | 2008-10-21 |
Identification of Phenylethanoid Glycosides in Plant Extract of Plantago asiatica L. by Liquid Chromatography‐Electrospray Ionization Mass Spectrometry |
| |
| Li LI,Chunming LIU,Zhiqiang LIU,Rong TSAO,Shuying LIU.Identification of Phenylethanoid Glycosides in Plant Extract of Plantago asiatica L. by Liquid Chromatography‐Electrospray Ionization Mass Spectrometry[J].Chinese Journal of Chemistry,2009,27(3):541-544. |
| |
| Authors: | Li LI Chunming LIU Zhiqiang LIU Rong TSAO Shuying LIU |
| |
| Institution: | 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China;2. The Central Laboratory, Changchun Normal University, Changchun, Jilin 130032, China;3. Tel.: 0086‐0431‐85262236;4. Fax: 0086‐0431‐85262886;5. Food Research Program, Agriculture & Agri‐Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada |
| |
| Abstract: | The present work describes a liquid chromatography‐electrospray ionization mass spectrometry (LC‐ESI‐MS) method for rapid identification of phenylethanoid glycosides in plant extract from Plantago asiatica L. By using a binary mobile phase system consisting of 0.2% acetic acid and acetonitrile under gradient conditions, a good separation was achieved on a reversed‐phase C18 column. The M–H]? ions, the molecular weights, and the fragment ions of phenylethanoid glycosides were obtained in the negative ion mode using LC‐ESI‐MS. The identification of the phenylethanoid glycosides (peaks 1–3) in the extract of P. asiatica L. was based on matching their retention time, the detection of molecular ions, and the fragment ions obtained by collision‐induced dissociation (CID) experiments with those of the authentic standards and data reported in the literature. |
| |
| Keywords: | Plantago asiatica L phenylethanoid glycoside liquid chromatography‐electrospray ionization mass spectrometry |
|
| 点击此处可从《中国化学》浏览原始摘要信息 |
| 点击此处可从《中国化学》下载免费的PDF全文 |
|
