Optimization of the extraction for the determination of arsenic species in plant materials by high-performance liquid chromatography coupled with hydride generation atomic fluorescence spectrometry |
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| Affiliation: | 1. Nuclear Physics Institute, Academy of Sciences of the Czech Republic, 250 68 Řež, Czech Republic;2. Department of Physics and Measurements, The University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic;3. Department of Physics, Jan Evangelista Purkyně University in Ústí nad Labem, České mládeže 8, 400 96 Ústí nad Labem, Czech Republic;4. Department of Condensed Matter Physics, Faculty of Mathematics and Physics, Charles University in Prague, Ke Karlovu 5, 121 16 Prague 2, Czech Republic;1. Department of Materials Science and Engineering, Ocean University of China, 266100, Qingdao, Peoples Republic of China, People’s Republic of China;2. Department of chemistry, Imperial College London, SW7 2AZ, London, UK;1. Department of Chemistry, Brandon University, Brandon, Manitoba R7A 6A9, Canada;2. Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada;3. Department of Chemistry “Ugo Schiff” and INSTM Research Unit of Florence, University of Florence, Sesto Fiorentino, Italy;4. Department of Applied Science and Technology, “Guglielmo Marconi” University, via Plinio 44, 00193 Roma, Italy;1. Research Center of Geotechnical and Structural Engineering, Shandong University, Jinan 250061, China;2. School of Resource Science, Hebei University of Engineering, Handan 056038, China;3. Jiangsu Key Laboratory of Resources and Environmental Information Engineering, NASG Key Laboratory of Land Environment and Disaster Monitoring, China University of Mining and Technology, Xuzhou 221116, China |
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| Abstract: | A study has been conducted for the separation and the determination of arsenic species in plants using high-performance liquid chromatography–hydride generation atomic fluorescence spectrometry with emphasis on sample extraction procedures. Various extraction solvents have been applied to extract arsenic species from plants in order to investigate the uptake, transfer and accumulation processes of arsenic. The method was optimized with respect to the selection of extraction solvent, extraction time and the number of extraction steps. The analytical procedure has been validated by analyzing standard reference material GBW 82301 (peach leaves) and successfully used for the arsenic speciation in plants grown on contaminated soil near an arsenic mine. Inorganic arsenic, especially arsenate (As(V)) appears to be the major component in plants and organic arsenic species of monomethylarsenic acid and dimethylarsenic acid were detected at low concentrations. |
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