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Determination of iron,cobalt, nickel,manganese, zinc,copper, cadmium and lead in human hair by inductively coupled plasma-atomic emission spectrometry
Affiliation:1. Department of Environmental Sciences, S.V. University, Tirupati 517 502, India;2. Department of Chemistry, S.V. University, Tirupati 517 502, India;3. Inorganic and Analytical Chemistry Group, Regional Research Laboratory, Trivandrum 695 019, India;4. Radio Chemistry Division, B.A.R.C., Mumbai 400 085, India;1. Vascular Unit, 2nd Clinic of Surgery, Aretaieion Hospital Medical School, University of Athens, Greece;2. 1st Clinic of Neurology, Aiginiteion Hospital;3. 1st Laboratory of Radiology, Aretaieion Hospital, Medical School, University of Athens, Greece;4. 1st Clinic of Anaesthesiology, Aretaieion Hospital, Medical School, University of Athens, Greece;1. Student Research Committee, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Environmental Health Engineering, Faculty of Health and Nutrition, Bushehr University of Medical Sciences, Bushehr, Iran;3. Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;4. Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;5. Department of Biostatistics, Faculty of Health and Nutrition, Bushehr University of Medical Sciences, Bushehr, Iran;6. Systems Environmental Health and Energy Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran;7. The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran;8. Department of Occupational Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran;9. Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran;1. Department of Chemistry, Hazara University, Mansehra 21120, Khyber Pukhtankhwa, Pakistan;2. Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, KPK, Pakistan;3. Department of Biochemistry and Biotechnology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan;4. Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), PuncakAlam Campus, 42300 Bandar PuncakAlam, Selangor DarulEhsan, Malaysia;5. Faculty of Applied Science, UiTM, 40450 Shah Alam, Selangor, Malaysia;6. Department of Environmental Science, COMSATS Institute of Information Technology, Abbottabad 22060, KPK, Pakistan
Abstract:A method was standardized for the dissolution of hair samples and analysis was carried out by inductively coupled plasma atomic emission spectrometry (ICP-AES). Hair samples were brought into solution by using a mixture of nitric acid and hydrogen peroxide. Various parameters that influence the sample preparation, namely temperature, digestion time and ratio of acid mixture were studied and standardized. The optimized method has been employed to digest standard reference materials and hair samples of residents of India, collected from different age groups and sex, and analyzed for Fe, Co, Ni, Mn, Zn, Cu, Cd and Pb. The values agree for most of the metals with the data reported for human hair samples of residents of India. The NIES CRM Human Hair No. 5 and IAEA Reference Hair HH-1 certified reference materials were used in order to verify the accuracy of the method and the results were in excellent agreement with the certified values.
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