| Studies of Dielectrophoretic Spectra of Levitated Tobacco Leaf Protoplast |
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| 作者姓名： | 汪和睦 杭军 |
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| 作者单位： | Physics Department，Nankai University，Tianjin，300071，PRC，Physics Department，Nankai University，Tianjin，300071，PRC |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | In this paper, we have utilized the principle of dielectrophoresis to determine the dielectrophoretic spectra of single levitated tobacco leaf protoplast at various suspending media conductivities (12.5 μS/cm-520 μS/cm) over wide frequencies (10 Hz—15 MHz). From the experimental data, the membrane capacitance of the single protoplast which was 0.40±0.03μF/cm~2 has been obtained and the dipole interaction of two protoplasts in electrofusion has also been analysed. When the external electric field E_c=1.5kV/cm was applied and the frequency was higher than 20 kHz, the dipole interaction pressure ~πd would be much larger than the membrane potential pressure ~πm, and the pressure ~πd could be the main reason for membraue pores. This conclusion will provide a new basis to improve the protocol of cell electrofusion.
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Studies of Dielectrophoretic Spectra of Levitated Tobacco Leaf Protoplast |
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| WANG He-Mu and HANG Jun.Studies of Dielectrophoretic Spectra of Levitated Tobacco Leaf Protoplast[J].Science in China(Chemistry),1993(9). |
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| Authors: | WANG He-Mu and HANG Jun |
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| Abstract: | |
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| Keywords: | plant protoplast levitated dielectrophoresis(DEP) membrane capacitance dipole interactions of cells |
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