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Cholesteric bonded stationary phases for high-performance liquid chromatography: a comparative study of the chromatographic behavior
Authors:Cédric Courtois  Guilhem Pagès  Stefano Caldarelli  Corinne Delaurent
Institution:1. Ism2, Aix Marseille Université, Case 512, Campus de Saint Jér?me, Avenue Escadrille Normandie-Niemen, 13397, Marseille Cedex 20, France
2. School of Molecular & Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
Abstract:The properties of four cholesteric bonded stationary phases differing in the nature of the spacer and the end-capping were assessed using simple chromatographic tests based on the retention of nonpolar compounds and of planar or nonplanar probe solutes. All cholesteric columns showed a hydrophobicity close to that of conventional octadecyldimethylsilyl (ODS) materials. Non-end-capped cholesteric bonded phases showed greater selectivity than ODS ones and both end-capped cholesteric bonded phases exhibit behavior intermediate between that of the non-end-capped original material and that of the ODS bonded phase.
Keywords:High-performance liquid chromatography  Column characterization  Cholesteric bonded stationary phase
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