中药白芍的HPLC含量测定及指纹图谱研究

建立白芍商品药材指纹图谱,并测定芍药苷含量评价药材质量。采用高效液相色谱分析,HypersilODS C18柱(4.6mm×250mm,5μm),乙腈-0.08%磷酸溶液梯度洗脱,柱温30℃。测定了市售17批白芍药材的HPLC指纹图谱,建立了白芍药材的总共有模式,计算得到各批药材的相似度,其中8批药材的相似度介于0.902—0.985。以相似度0.90为界,大于0.90认定为合格药材,小于0.90为不合格药材。本评价方法的结果与根据药典标准的划分结果一致,但更直观,可操作性更强,尤其对白芍药材中硫化物进行有效监控,还可对熏硫药材的质量控制起到示范作用。

Study of the Content of Paeoniflorin and HPLC Fingerprint in Radix Paeoniae Alba

A HPLC-fingerprint of Radix Paeoniae Alba was established and the drug's quality was evaluated by determining the content of paeoniflorin.Analysis was performed using a Hypersil ODS C_(18) column (4.6mm×250mm, 5μm) with the mixture of acetonitrile-0.08% phosphoric acid solution as mobile phase in gradient mode, and the column temperature was at 30℃.Seventeen samples of the crude drugs of Radix Paeoniae Alba were determined.The mutual model was established and the similarity degrees were calculated.Similarity degrees of 8 samples were between 0.902-0.985 respectively.The limit of similarity degree for Radix Paeoniae Alba was set at 0.90.If the similarity degree was greater than 0.90,it was qualified crude drug,otherwise it was unqualified.The evaluation results from this method were the same as the results from the method published in the Chinese Pharmacopoeia (2010ed.) and it can be used to control the sulfide contended in Radix Paeoniae Alba.