用于赭曲霉毒素A检测的电化学适配体生物传感器的研究进展

赭曲霉毒素(Ochratoxin)是一类主要由曲霉菌和青霉菌产生的次生代谢产物,其中赭曲霉毒素A(OTA)的毒性最强。OTA相当稳定,常规的食品加工难以去除,若摄入受OTA污染的食品或药物会对人类造成严重的危害。实现对OTA的灵敏和快速检测是及早发现和处置OTA污染的关键。近年来,核酸适配体因其独特的优点,被作为抗体的替代物用于构建OTA电化学生物传感器。本文介绍了经典的OTA检测方法和基于适配体的电化学生物传感检测方法,从OTA电化学适配体传感器的适配体优化、新型材料应用以及生物信号放大技术的应用等三个方面总结了该生物传感技术的研究现状,并对其未来的发展进行了展望。

Progress in Electrochemical Aptamer Biosensors for the Detection of Ochratoxin A

Ochratoxin is a kind of secondary metabolites mainly produced by aspergillus and penicillium. Ochratoxin A (OTA) is the most toxic one which can cause serious harm to human body. Moreover, it is difficult to be destroyed by conventional food processing because of its high stability. Sensitive and rapid detection of OTA is the key to early detection and treatment of OTA contamination. So far, aptamers have been employed as substitutes for antibodies to construct OTA electrochemical biosensors due to their unique advantages. The classic OTA detection methods and electrochemical aptamer biosensors (aptasensors) were introduced respectively in this paper. Besides, the latest research status of the OTA electrochemical aptasensors were summarized including the optimization of aptamer, the applications of new materials and biomagnification technologies. Meantime, the future was prospected with the aim of providing some reference for the further research and application of OTA aptasensors.