光纤化学传感同步吸收-荧光法的建立

建立一种光纤化学传感同步测定吸收光谱和荧光光谱的新方法。自制同步吸收-荧光比色皿，结合光纤化学传感技术，建立同步吸收-荧光光谱法检测仪器，分别测定罗丹明B，维生素B₂，维生素B₆等溶液的同步吸收-荧光光谱，且与传统的紫外-可见吸光光度法和荧光法进行对比。同步吸收-荧光光谱法测得罗丹明B，维生素B₂，维生素B₆与传统吸光光度法和荧光光谱法检测的吸收光谱图和荧光光谱的图谱大体一致。最大荧光强度波长与传统的荧光方法比较准确度高，但最大吸收波长略有偏差。同步吸收-荧光光谱法等同于同步测定荧光物质的吸收光谱和荧光光谱，实现二光谱合二为一，测定最大发射波长时准确度高，但最大吸收波长略有偏差，值得深一步研究。

The Establishment of the Method of the Fiber Optic Chemical Sensor Synchronous Absorption-Fluorescence

A new method of simultaneously measuring fiber-optic chemical sensor absorption spectrum and fluorescence spectrum is established. Make synchronous absorption-fluorescence cuvette, establish synchronous absorption-fluorescence spectrometry instrumentation combined by fiber optic chemical sensor technology, measure the synchronous absorption-fluorescence spectrums of solutions of rhodamine B, vitamin B₂ and vitamin B₆, compared by absorption spectroscopy measured by traditional UV-Visible photometric method and fluorescence spectroscopy measured by traditional fluorescence method. Synchronous absorption-fluorescence method measure absorption spectrums and fluorescence spectrums the same to traditional photometric and fluorescence spectroscopy of rhodamine B, vitamin B₂ and vitamin B₆. The maximum wavelength of fluorescence intensity method has high accuracy relatively compared with fluorescence, but the maximum wavelength of absorption has a slight deviation. Synchronous absorption-fluorescence method means simultaneously measure the absorption spectrums and fluorescence spectrums of the fluorescent substance, making two spectrums to one. The method measured the maximum emission wavelength with high accuracy, though in measuring maximum absorption wavelength there is a slight deviation, but it is worth further studying.