| DNA microarray synthesis by using PDMS molecular stamp (Ⅱ)——Oligonucleotide on-chip synthesis using PDMS stamp |
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| 引用本文： | 肖鹏峰,何农跃,贺全国,张春秀,王轶文,陆祖宏,徐吉庆. DNA microarray synthesis by using PDMS molecular stamp (Ⅱ)——Oligonucleotide on-chip synthesis using PDMS stamp[J]. 中国科学B辑(英文版), 2001, 44(4) |
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| 作者姓名： | 肖鹏峰 何农跃 贺全国 张春秀 王轶文 陆祖宏 徐吉庆 |
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| 基金项目： | Acknowledgements This work was supported by the National 863 High-Tech Project, 973 National Key Fundamental Research Project and the National Natural Science Foundation of China (Grant No. 60071001). |
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| 摘 要： | Based on the standard phosphoramidites chemistry protocol, two oligonucleotides synthetic routes were studied by contact stamping reactants to a modified glass slide. Route A was a contact coupling reaction, in which a nucleoside monomer was transferred and coupled to reactive groups (OH) on a substrate by spreading the nucleoside activated with tetrazole on a polydimethylsiloxane (PDMS) stamp. Route B was a contact detritylation, in which one nucleoside was fixed on the desired synthesis regions where dimethoxytrityl (DMT) protecting groups on the 5'-hydroxyl of the support-bound nucleoside were removed by stamping trichloroacetic acid (TCA) distributed on features on a PDMS stamp. Experiments showed that the synthetic yield and the reaction speed of route A were higher than those of route B. It was shown that 20 mer oligonucleo-tide arrays immobilized on the glass slide were successfully synthesized using the PDMS stamps, and the coupling efficiency showed no difference between the PDMS stamping and the co
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DNA microarray synthesis by using PDMS molecular stamp (II) -- Oligonucleotide on-chip synthesis using PDMS stamp |
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| Xiao Pengfeng,He Nongyue,HE Quanguo,Zhang Chunxiu,Wang Yiwen,Lu Zuhong,XU Jiqing. DNA microarray synthesis by using PDMS molecular stamp (II) -- Oligonucleotide on-chip synthesis using PDMS stamp[J]. Science in China(Chemistry), 2001, 44(4) |
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| Authors: | Xiao Pengfeng He Nongyue HE Quanguo Zhang Chunxiu Wang Yiwen Lu Zuhong XU Jiqing |
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| Affiliation: | 1. Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University,
2. Nanjing E-life Gene Medicine Ltd. |
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| Abstract: | Based on the standard phosphoramidites chemistry protocol, two oligonucleotides synthetic routes were studied by contact stamping reactants to a modified glass slide. Route A was a contact coupling reaction, in which a nucleoside monomer was transferred and coupled to reactive groups (OH) on a substrate by spreading the nucleoside activated with tetrazole on a polydimethylsiloxane (PDMS) stamp. Route B was a contact detritylation, in which one nucleoside was fixed on the desired synthesis regions where dimethoxytrityl (DMT) protecting groups on the 5'-hydroxyl of the support-bound nucleoside were removed by stamping trichloroacetic acid (TCA) distributed on features on a PDMS stamp. Experiments showed that the synthetic yield and the reaction speed of route A were higher than those of route B. It was shown that 20 mer oligonucleo-tide arrays immobilized on the glass slide were successfully synthesized using the PDMS stamps, and the coupling efficiency showed no difference between the PDMS stamping and the conventional synthesis methods. |
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| Keywords: | PDMS stamps contact coupling contact detritylation oligonucleotide synthesis. |
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