A colorimetric and ratiometric fluorescent probe for quantification of bovine serum albumin |
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Authors: | Zeng Xiaodan Zhang Xiaoling Zhu Baocun Jia Hongying Li Yamin Xue Juan |
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Institution: | Key Laboratory of Cluster Science of Ministry of Education, Department of Chemistry, School of Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China. |
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Abstract: | A 4-aminonaphthalimide-based ratiometric fluorescent probe 1 employing the internal charge transfer (ICT) mechanism was designed and synthesized to detect bovine serum albumin (BSA). The interaction of 1 and BSA was investigated by fluorescence and UV-vis absorption spectroscopy. Upon treatment with BSA, the probe successfully exhibited a ratiometric fluorescent response at 540 nm and 480 nm. The fluorescent intensity ratio at 540 nm and 480 nm (F(540)/F(480)) increases linearly with BSA concentration in the range of 0-75.0 μg mL(-1) and the detection limit was about 2.4 ng mL(-1). Our strategy is expected to provide a methodology to quantify BSA either by a normal or by a ratiometric and colorimetric way with high sensitivity. |
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