| Compatibility of Chitosan-Gelatin Films with Adipose Tissue Derived Stromal Cells |
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| 作者姓名： | 张玲 高媛 孔丽君 公衍道 赵南明 张秀芳 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China |
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| 基金项目： | Supported by the National Key Basic Research and Development (973) Program of China (No. 2005CB623905), the National Natural Science Foundation of China (No. 30400099), and the Tsinghua-Yue-Yuen Medical Science Fund |
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| 摘 要： | Introduction Chitosan, the partially N-deacetylated form of chitin, is the second most abundant biomaterial after cellulose. Due to its biocompatibility, biodegradability, and bio- activity, chitosan and its derivant complexes have been studied in various…
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| 关 键 词： | 壳聚糖 凝胶 ADSCs 组织工程 相容性 |
| 收稿时间： | 2005-09-20 |
| 修稿时间： | 2005-09-202005-10-20 |
Compatibility of Chitosan-Gelatin Films with Adipose Tissue Derived Stromal Cells |
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| ZHANG Ling,GAO Yuan,KONG Lijun,GONG Yandao,ZHAO Nanming,ZHANG Xiufang.Compatibility of Chitosan-Gelatin Films with Adipose Tissue Derived Stromal Cells[J].Tsinghua Science and Technology,2006,11(4):421-426. |
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| Authors: | ZHANG Ling GAO Yuan KONG Lijun GONG Yandao ZHAO Nanming ZHANG Xiufang |
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| Institution: | 1. Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran;2. Department of Mechanical Engineering, Shahid Bahonar University of Kerman, Kerman, Iran;3. Department of Engineering Design and Materials, NTNU, Trondheim, Norway;1. Institute of Theoretical Physics, Xinyang Normal University, Xinyang, Henan 464000, China;2. Institute of Particle Physics and Key Laboratory of Quark and Lepton Physics (MOE), Central China Normal University, Wuhan, 430079, China;3. Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA;1. Department of Physics and Astronomy, University of California, Irvine, CA 92697-4575, USA;2. Instituto de Física, Universidad Nacional Autónoma de México, POB 20-364, Cd.Mx. 01000, Mexico;3. Physik Department T75, Technische Universität München, James-Franck-Straße 1, 85748, Garching, Germany;1. Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei, Taiwan;2. Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan;3. Department of Biotechnology, Asia University, Taichung, Taiwan;4. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan;5. Institute of Clinical and Community Health Nursing, National Yang-Ming University, Taipei, Taiwan;6. Department of Obstetrics and Gynecology, School of Medicine, National Yang-Ming University, Taipei, Taiwan;7. Genephile Bioscience Laboratory, Ko''s Obstetrics and Gynecology, Taipei, Taiwan;8. Department of Pediatrics, MacKay Memorial Hospital, Taipei, Taiwan;9. Department of Medicine, MacKay Medical College, New Taipei City, Taiwan;10. Department of Early Childhood Care, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan;11. Gene Biodesign Co. Ltd, Taipei, Taiwan;12. Department of Bioengineering, Tatung University, Taipei, Taiwan |
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| Abstract: | Chitosan has been shown to be a promising material for various applications in tissue engineering. Recently, adipose tissue derived stromal cells (ADSCs) have been investigated as an alternative source of seed cells for tissue engineering. The compatibility of chitosan and chitosan-gelatin complexes with ADSCs is not known. In the present study, ADSCs were isolated and characterized by phenotype using fluores- cence-activated cell sorting (FACS). The morphology, viability, and the ability of the ADSCs to differentiate on chitosan and chitosan-gelatin composite films with 60 wt.% gelatin were evaluated. Results show that the ADSCs are positive for CD29, CD44, and CD105, but negative for CD31, CD34, and CD45. ADSCs adhere and grow better on the composite films than on the chitosan films. The ability of ADSCs to differentiate into osteogenic and adipogenic lineage cells is not affected by their being cultured on chitosan-gelatin composite films. Therefore, chitosan-gelatin composite films are compatible with ADSCs and do not impair the ability of ADSCs to differentiate into osteogenic and adipogenic lineage cells. |
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| Keywords: | chitosan gelatin adipose tissue derived stromal cells (ADSCs) compatibility |
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