纳米金修饰电流型CA19-9免疫传感器的制备及应用

通过固定辣根过氧化酶（HRP）标记CA19-9抗体在纳米金修饰的碳糊电极制备了CA19-9安培免疫传感器。该免疫传感器在舍有CA19-9抗原的磷酸盐缓冲溶液中培育后，溶液中CA19-9抗原分子和HRP标记CA19-9抗体分子免疫结合导致了传感器电流的降低。在优化的实验条件下，样品中CA19-9浓度在2～30U／mL范围内与电流降低成线性关系，方法的检出限为1．4U／mL。该免疫传感器表现出较好的稳定性、准确性和重现性，为临床免疫分析提供了一种快速便捷方法。

Preparation and application of colloidal gold modified amperometric CA 19-9 immunosensor

A novel immunosensor for the determination of carcinoma antigen-19-9(CA19-9) is made by immobilization of horseradish peroxidase(HRP) labeled CA19-9 antibody on colloidal gold carbon paste electrodes.The incubation of the immunosensor in 0.1 M pH 7.0 PBS containing CA19-9 antigen was attributed to the formation of antigen-antibody complex,which blocked the electron transfer of HRP toward the electrode and resulted in significant decrease of peak current.Under optimal conditions,the decrease of the current is proportional to CA 19-9 concentration ranging from 2 to 30 U/mL with a detection limit of 1.4 U/mL.The CA 19-9 immunosensor shows a good accuracy and an acceptable precision and fabrication reproducibility.The proposed method provides a new promising platform for clinical immunoassay.