| 高效液相色谱与质谱联用分析测定川芎中有效成分阿魏酸与藁本内酯 |
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| 引用本文： | 孔亮,于志远,邹汉法,孙乃昌,吴丽华,倪坚毅.高效液相色谱与质谱联用分析测定川芎中有效成分阿魏酸与藁本内酯[J].分析化学,2004,32(11):1501-1504. |
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| 作者姓名： | 孔亮 于志远 邹汉法 孙乃昌 吴丽华 倪坚毅 |
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| 作者单位： | 中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116011 |
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| 基金项目： | 国家自然科学基金资助项目 (No .90 2 0 90 5 6) |
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| 摘 要： | 采用HPLC-MS系统地研究了中药川芎有效成分阿魏酸(ferykuc acid)和藁本内酯(liguatilide)的定量分析方法。利用制备色谱制备了藁本内酯对照品，并对其进行了紫外光谱、质谱、红外光谱等结构鉴定。分别考察了水、甲醇、乙醇、95％乙醇4种溶剂以及提取时间对川芎中阿魏酸和藁本内酯提取量的影响。结果表明：水是阿魏酸的最佳提取溶剂，提取时间45min为宜；乙醇是适合提取藁本内酯的溶剂，提取时间75min为宜。以外标法对市售川芎中的阿魏酸与藁本内酯进行了定量分析，二者含量分别是0．15％(m／m)和0．82％(m/m)。
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| 关 键 词： | 高效液相色谱与质谱联用 川芎 质量控制 阿魏酸 藁本内酯 |
Determination of the Active Ingredients in Rhizoma Chuanxiong by High Performance Liquid Chromatography-Mass Spectrometry |
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| Kong Liang,Yu Zhiyuan,Zou Hanfa ,Sun Naichang,Wu Lihua,Ni Jianyi.Determination of the Active Ingredients in Rhizoma Chuanxiong by High Performance Liquid Chromatography-Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2004,32(11):1501-1504. |
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| Authors: | Kong Liang Yu Zhiyuan Zou Hanfa Sun Naichang Wu Lihua Ni Jianyi |
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| Institution: | Kong Liang,Yu Zhiyuan,Zou Hanfa *,Sun Naichang,Wu Lihua,Ni Jianyi |
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| Abstract: | The main active ingredients, ferulic acid and ligustilide in Chinese traditional medicine, Rhizoma Chuanxiong , were separated and identified by high performance liquid chromatography mass sepctrum (HPLC MS). The standard ligustilide was prepared with preparative scale HPLC and identified by HPLC diode array detection, mass spectrometry, infrared spectroscopy. Four kinds of extractiting solvent and extraction time affecting on the extraction efficiency of ferulic acid and ligustilide in Rhizoma Chuanxiong were examined, and a quality control method was also developed based on the quantitative determination of ferulic acid and liguistilide in extracted solutions of Rhizoma Chuanxiong by water and ethanol. The amount of ferulic acid and ligustilide in Rhizoma Chuanxiong determined are as much as 0.15% ( W/W ) and 0.82% ( W/W ). |
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| Keywords: | High performance liquid chromatography mass spectrometry Rhizoma Chuanxiong quality control ferulic acid ligustilide |
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