Range of separation of potential tool for bioseparation, microchip electrophoresis system, for DNA polymorphisms on the human Y-chromosome. |
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| Authors: | Mohammad Jabasini Feng Xu Fuquan Dang Toshikatsu Shinka Yutaka Nakahori Yoshinobu Baba |
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| Affiliation: | Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation, Tokushima 770-8505, Japan. c400141005@stud.tokushima-u.ac.jp |
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| Abstract: | For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp to 189 bp was successfully separated with high resolution. All of these results demonstrate the superiority of microchip electrophoresis as a tool for 21st century bioseparation. |
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