Antibacterial Mode of Action of the Daucus carota Essential Oil Active Compounds against Campylobacter jejuni and Efflux-Mediated Drug Resistance in Gram-Negative Bacteria |
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Authors: | Dedieu Luc Brunel Jean Michel Lorenzi Vanina Muselli Alain Berti Liliane Bolla Jean Michel |
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Institution: | 1.Avignon Univ, INRAE, UMR SQPOV, SporAlim Team, Domaine Saint-Paul–Site Agroparc, 84914 Avignon CEDEX 9, France; ;2.Aix-Marseille Univ, INSERM, SSA, IRBA, MCT, UMR_MD1, U-1261, 13005 Marseille CEDEX 5, France; ;3.Université de Corse, UMR CNRS 6134, SPE Sciences Pour l’Environnement, Campus Grimaldi, 20250 Corte, France; (L.V.); (M.A.); (B.L.) |
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Abstract: | Today, an alarming rise of bacterial gastroenteritis in humans resulting from consuming Campylobacter-tainted foods is being observed. One of the solutions for mitigating this issue may be the antibacterial activity of essential oils. In the present research, we propose to study the antibacterial activity against Campylobacter and other Gram-negative bacteria of Daucus carota essential oil and its active molecules. In addition, a few chemically synthesized molecules such as (E)-methylisoeugenol, Elemicin, and eugenol were also studied. The results showed that the essential oil itself and its most active component, (E)-methylisoeugenol, exhibited bactericidal effects. Similar effects were detected using purified and chemically synthesized molecules. Also, it was observed that the Daucus carota essential oil and its active molecules affected intracellular potassium and intracellular ATP contents in Campylobacter cells. Inhibition of the membrane bound FOF1-ATPase was also observed. Eventually, for the first time, the efflux mechanism of active molecules of Daucus carota essential oil was also identified in gamma proteobacteria and its specific antibacterial activity against Campylobacter jejuni was associated with the lack of this efflux mechanism in this species. |
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Keywords: | natural compounds chemical synthesis efflux pump ATP monitoring Elemicin E-methylisoeugenol |
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