Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using (153) Eu Isotope Dilution ICP/MS |
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| Authors: | Yan Xiaowen Luo Yacui Zhang Zhubao Li Zhaoxin Luo Qiang Yang Limin Zhang Bo Chen Haifeng Bai Peiming Wang Qiuquan |
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| Institution: | Department of Chemistry and the Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China). |
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| Abstract: | Click and analyze: The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153) Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153) Eu(NO(3) )(3) isotopic standard. |
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| Keywords: | europium isotopic labeling liquid chromatography mass specrometry proteins |
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