Effect
of ethanol on the thermal stability of human serum albumin |
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| Authors: | Anna Michnik Zofia Drzazga |
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| Institution: | (1) A. Chełkowski’ Institute of Physics, Department of Medical Physics, University of Silesia, Katowice, Poland, 40-007, ul. Uniwerytecka 4 |
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| Abstract: | The effect of ethanol on human serum albumin stability in aqueous solution
was studied by use of differential scanning calorimetry. A deconvolution of
DSC traces in 2-state model with ΔC
p=0
and ΔC
p≠0
was performed and analysed to obtain information on the interaction of ethanol
with different parts of albumin molecule both fatty acid containing and fatty
acid free. The differences in ethanol binding affinity for both kinds of albumin
were found. At very low concentrations ethanol was observed to be a stabilizer
of the folded state of albumin contrary to the higher concentration where
its binding to the unfolded protein predominates. |
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| Keywords: | albumin– ethanol interactions DSC human serum albumin thermal stability |
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