| QUANTITATIVE RELATION BETWEEN MODIFICATION OF FUNCTIONAL GROUPS OF PROTEINS AND THEIR BIOLOGICAL ACTIVITY——A COMPUTER PROGRAM FOR ASCERTAINING THE NUMBER OF ESSENTIAL RESIDUES |
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| 引用本文： | 孙之荣,赵康源,王志新,周海梦,邹承鲁.QUANTITATIVE RELATION BETWEEN MODIFICATION OF FUNCTIONAL GROUPS OF PROTEINS AND THEIR BIOLOGICAL ACTIVITY——A COMPUTER PROGRAM FOR ASCERTAINING THE NUMBER OF ESSENTIAL RESIDUES[J].中国科学B辑(英文版),1992(11). |
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| 作者姓名： | 孙之荣 赵康源 王志新 周海梦 邹承鲁 |
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| 作者单位： | Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100101，PRC，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100101，PRC，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，National Laboratory of Biomacromolecules，Institute of Biophysics，Academia Sinica，Beijing 100101，PRC |
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| 摘 要： | Based on a statistical treatment of the quantitative relation between the extents of mod-ification of protein functional groups and the decrease of their biological activity, a meth-od was proposed (Tsou, Sci. Sin., 1962, 11:1535) and widely used for the determination ofthe number of residues essential for the biological activity ot the protein modified. How-ever, the original method depends on a trial and error approach and manual calculation tofind the best fit for the data obtained. A computer program is described in this paper forthe treatment of experimental data to produce the number of essential and non-essentialgroups modified and the ratio of rates for their modification which best fit the data ob-tained. Applications of this method show that in some cases wrong conclusions were reachedin literature and in other cases quantitative conclusions can sometimes be drawn when orig-inally either no quantitative data treatment was presented or attempts at manual curve fit-ting were unsuccessful.
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