Production of single-cell protein with two-step fermentation for treatment of potato starch processing waste |
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| Authors: | Bingnan Liu Ying Li Jinzhu Song Lihong Zhang Jiacheng Dong Qian Yang |
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| Institution: | 1. Bioengineering Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, People’s Republic of China
2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China
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| Abstract: | Potato starch processing waste is causing serious environmental problems. This study aimed to convert potato starch processing waste into single-cell protein as high-quality feed using a two-step fermentation process. The mutant strain Aspergillus niger H3 was selected after UV irradiation and ethyl methyl sulfone mutagenesis for more cellulase production. The activities of sodium carboxymethyl cellulase and filter paperase of strain H3 were 8.86 and 4.79 U, respectively, which were much higher than the parent strain (1.18 and 0.62 U). After treatment with strain H3, the cellulose degradation rate of potato residue was 80.54 %. A liquid fermentation using Bacillus licheniformis was performed as the second step. The optimized fermentation conditions were temperature of 32.8 °C, pH 6.67, and inoculum concentration of 1.78 % using the response surface method. Results of this study showed a potential application in large-scale industrial conversion. |
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