Enhancing enzymatic hydrolysis of crystalline cellulose and lignocellulose by adding long-chain fatty alcohols |
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Authors: | Hongming Lou Huanran Lai Shun Wu Xiuli Li Dongjie Yang Xueqing Qiu Jinhao Huang Conghua Yi |
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Affiliation: | 1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China 2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China
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Abstract: | The effects of long-chain fatty alcohols (LFAs) on the enzymatic hydrolysis of crystalline cellulose by two commercial Trichoderma reesei cellulase cocktails (CTec2 and Celluclast 1.5L) were studied. It was found that n-butanol inhibited the enzymatic hydrolysis, but n-octanol, n-decanol and n-dodecanol had strong enhancement on enzymatic hydrolysis of crystalline cellulose in the buffer pH range from 4.0 to 6.0. LFAs can increase the hydrolysis efficiency of crystalline cellulose from 37 to 57 % at Celluclast 1.5L loading of ten filter paper units (FPU)/g glucan. LFAs have similar enhancement on the enzymatic hydrolysis of crystalline cellulose mixed with lignin or xylan. The enhancement of LFAs increased with the decrease of the crystallinity index. LFAs not only enhanced the high-solid enzymatic hydrolysis of lignocellulose, but also improved the rheological properties of high-solid lignocellulosic slurries by decreasing the yield stress and complex viscosity. Meanwhile, LFAs can improve the enzymatic hydrolysis of cellobiose to glucose, especially at low cellulase loading. |
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