Selective chemical imaging of static actin in live cells |
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Authors: | Milroy Lech-Gustav Rizzo Stefano Calderon Abram Ellinger Bernhard Erdmann Silke Mondry Justine Verveer Peter Bastiaens Philippe Waldmann Herbert Dehmelt Leif Arndt Hans-Dieter |
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Institution: | Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany. |
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Abstract: | We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics. |
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