| Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis |
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| 作者单位： | Yong Nian Ni（Department of Chemistry, Nanchang University, Nanchang 330047, China;The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China） ;
Xue Zhi Zhong（Department of Chemistry, Nanchang University, Nanchang 330047, China） ; |
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| 基金项目： | 国家自然科学基金;江西省自然科学基金 |
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| 摘 要： | The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6 × 104 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.
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| 关 键 词： | DNA 中性红 相互作用 荧光分光光谱分析 结合常数 |
| 收稿时间： | 2007-01-04 |
Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis |
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| Authors: | Yong Nian Ni Xue Zhi Zhong |
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| Institution: | 1. Department of Chemistry, Nanchang University, Nanchang 330047, China;2. The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China |
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| Abstract: | The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug–DNA binding process, and then the intrinsic binding constant of 1.6 × 104 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot. |
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| Keywords: | Neutral red DNA Binding constant Fluorescence Chemometrics Singular value decomposition |
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