| Derivatization Reaction of Carbohydrates with Urea as the Reagent and Fluorimetric Determination of Carbohydrates |
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| 作者姓名： | 杨景和 曹西慧 王敏 吴霞 孙长侠 |
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| 作者单位： | YANG，Jing-He CAO，Xi-Hui WANG，Min WU，Xia SUN，Chang-XiaKey Laboratory of Colloid & Interface Chemistry for Education Ministry，Department of Chemistry，Shandong University，Jinan，Shandong 250100，China |
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| 基金项目： | theNationalNaturalScienceFoundationsofChina (No .2 9875 0 15 ),theNaturalScienceFoundationofShandongProvince (No .Z97B0 10 0 2 ),andtheVisitingScholarFoundationofKeyLaboratoryinShandongUniversity |
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| 摘 要： | ＩｎｔｒｏｄｕｃｔｉｏｎＣａｒｂｏｈｙｄｒａｔｅｓｐｌａｙａｎｅｓｓｅｎｔｉａｌｒｏｌｅｉｎｎａｔｕｒｅａｎｄａｌｍｏｓｔａｌｌｔｈｅｌｉｆｅｐｒｏｃｅｓｓｅｓ .Ｔｈｅｙａｒｅｎｏｔｏｎｌｙｃｅｎｔｒａｌｔｏｔｈｅｇｅｎｅｒａｔｉｏｎａｎｄｓｔｏｒａｇｅｏｆｅｎｅｒｇｙｂｕｔａｌｓｏｕｓｅｆｕｌｉｎｔｈｅｄｉａｇｎｏｓｉｓｏｆｄｉｓｅａｓｅｓａｎｄｄｙｎａｍｉｃａｄｊｕｓｔｍｅｎｔｆｏｒｔｈｅｆｕｎｃｔｉｏｎｓｏｆｐｒｏｔｅｉｎ .Ｓｏｅｓｔａｂｌｉｓｈｉｎｇｈｉｇｈｓｅｎｓｉｔｉｖｅａｎｄｅｆｆ…
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| 关 键 词： | 碳水化合物 衍生反应 尿素 反应剂 荧光测定法 |
Derivatization Reaction of Carbohydrates with Urea as the Reagent and Fluorimetric Determination of Carbohydrates |
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| YANG,Jing-He CAO,Xi-Hui WANG,Min WU,Xia SUN,Chang-XiaKey Laboratory of Colloid & Interface Chemistry for Education Ministry.Derivatization Reaction of Carbohydrates with Urea as the Reagent and Fluorimetric Determination of Carbohydrates[J].Chinese Journal of Chemistry,2002,20(1):51-56. |
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| Authors: | YANG Jing-He CAO Xi-Hui WANG Min WU Xia SUN Chang-XiaKey Laboratory of Colloid & Interface Chemistry for Education Ministry |
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| Institution: | YANG,Jing-He CAO,Xi-Hui WANG,Min WU,Xia SUN,Chang-XiaKey Laboratory of Colloid & Interface Chemistry for Education Ministry,Department of Chemistry,Shandong University,Jinan,Shandong 250100,China |
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| Abstract: | It is found that in the presence of sulfuric add carbohydrates condense with urea to afford the condensation products, which emit fluorescence. Under optimum conditions, the fluorescence intensities of system are proportional to the concentrations of carbohydrates. Based on this linear relationship, quantitative determination of kinds of carbohydrates has been made. Among all the carbohydrates tested, the sensitivity of α‐rhamnose is the highest and its limits of detection reaches 3.5 × 10?8 mol/L. So α‐rhamnose can be selectively determined in the presence of other carbohydrates. A interaction mechanism is also discussed. |
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| Keywords: | carbohydrate urea derivatization reaction fluorimetric determination |
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