Development of reference materials for microbiological analysis |
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Authors: | Wolfgang J Philipp Pierre van Iwaarden Heinz Schimmel Nele Meeus Nadine Kollmorgen |
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Institution: | (1) European Commission, DG Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440 Geel, Belgium |
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Abstract: | The reliability of reference materials (RMs) depends on properties such as fitness, robustness, commutability, stability and
homogeneity. The development of RMs for microbiological analysis is especially challenged through questions around the stabilisation
and recovery of viable cells, the dispersion of precise numbers of cells, matrix effects and, when using molecular techniques,
the presence of nucleic acids (e.g. DNA) of dead and live target organisms. However, RMs are indispensable tools for quality
control in microbiological analysis. The Institute for Reference Materials and Measurements (IRMM), as part of the European
Commission, concentrates its efforts on the development of RMs to support the development, implementation and monitoring of
EU legislation. A special focus is given to highly precise RMs for presence/absence and enumeration tests in microbiological
food and water analysis. Another group of new RMs certified by the IRMM comprise DNA-based materials to control the identity
of micro-organisms in qualitative assays. All of these activities serve to improve quality control in microbiological analysis.
Presented at ‘BERM-10’, April 2006, Charleston, SC, USA. |
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Keywords: | Reference materials Microbiological analysis Quality control Food microbiology Water microbiology Pathogens |
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