| 高效液相色谱法对牦牛血浆与尿中嘌呤衍生物及肌酐含量的测定 |
| |
| 引用本文： | 李晓鹏,周围,王虎成,龙丹凤,周建伟,龙瑞军. 高效液相色谱法对牦牛血浆与尿中嘌呤衍生物及肌酐含量的测定[J]. 分析测试学报, 2009, 28(7). DOI: 10.3969/j.issn.1004-4957.2009.07.024 |
| |
| 作者姓名： | 李晓鹏 周围 王虎成 龙丹凤 周建伟 龙瑞军 |
| |
| 作者单位： | 甘肃农业大学,草业学院,甘肃,兰州,730070;兰州大学,青藏高原生态系统管理国际中心,甘肃,兰州,730020;甘肃省出入境检验检疫局,中心实验室,甘肃,兰州,730020;兰州大学,青藏高原生态系统管理国际中心,甘肃,兰州,730020;兰州大学,草地农业科技学院,甘肃,兰州,730020;兰州大学,青藏高原生态系统管理国际中心,甘肃,兰州,730020 |
| |
| 基金项目： | 国家自然科学基金重点项目 |
| |
| 摘 要： | 建立了牦牛血浆及尿中的嘌呤衍生物及肌酐含量测定的HPLC/UVD方法.在室温条件下,以乙酸铵为流动相,分别以Lichrospher RP-C18 Merck(4.6×250 mm,5 μm i.d.)和5C18-AR-Ⅲ Waters(4.6×250 mm)为色谱柱,检测波长220 nm,流速1 mL/min,对尿囊素、尿酸、肌酐、黄嘌呤和次黄嘌呤进行了检测.方法检出限(LOD)为0.2 ~0.4 μmol/L,血样和尿样的加标回收率分别为81% ~103%、83% ~104%.
|
| 关 键 词： | 高效液相色谱 嘌呤衍生物 肌酐 牛尿 牛血浆 |
Determination of Purine Derivatives and Creatinine in Yak's Urine and Plasma by HPLC |
| |
| LI Xiao-peng,ZHOU Wei,WANG Hu-cheng,LONG Dan-feng,ZHOU Jian-wei,LONG Rui-jun. Determination of Purine Derivatives and Creatinine in Yak's Urine and Plasma by HPLC[J]. Journal of Instrumental Analysis, 2009, 28(7). DOI: 10.3969/j.issn.1004-4957.2009.07.024 |
| |
| Authors: | LI Xiao-peng ZHOU Wei WANG Hu-cheng LONG Dan-feng ZHOU Jian-wei LONG Rui-jun |
| |
| Abstract: | A method of high performance liquid chromatography(HPLC) coupled with UVD was developed for the determination of purine derivatives(PD),including allantoin,creatinine,uric acid,xanthine and hypoxanthine,and creatinine in white compounds were achieved on Lichrospher RP-C18 Merck(4.6×250 mm,5 μm i.d.) and 5C18-AR-Ⅲ Waters(4.6×250 mm) columns,respectively,under the optimal conditions of using ammonium acetate as mobile phase with a flow rate of 1 mL/min at 25 ℃.The determination of the analytes was accomplished within 10 min.The limits of 83%-104%,respectively.The method was rapid,accurate and effective,and could be and urine sample. |
| |
| Keywords: | HPLC purine derivative creatinine |
| 本文献已被 万方数据 等数据库收录！ |
|
