Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering |
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Authors: | Yu-Fen Huang Yang-Wei Lin Zong-Hong Lin Huan-Tsung Chang |
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Institution: | (1) Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 106, Taiwan, ROC;(2) Department of Natural Science Education, National Taitung University, Taitung, Taiwan, ROC |
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Abstract: | In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for
cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and
Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells
(normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold
ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation
of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal
cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells,
but not H184B5F5/M10 cells. |
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Keywords: | Aptamer Gold nanoparticles Breast cancer cells Imaging Proliferation Nanomedicine |
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