Cytotoxicity and drug release behavior of PNIPAM grafted on silica-coated iron oxide nanoparticles |
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Authors: | Yi-Hsin Lien Tzong-Ming Wu Jhao-Huei Wu Jiunn-Wang Liao |
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Institution: | (1) Department of Materials Science and Engineering, National Chung Hsing University, 250, Kuo Kuang Road, 402 Taichung, Taiwan;(2) Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, 250, Kuo Kuang Road, 402 Taichung, Taiwan; |
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Abstract: | The nanoparticles containing thermosensitive and magnetic properties were investigated for their potential use as a novel
drug carrier for targeted and controlled release drug delivery system. These thermosensitive and magnetic nanoparticles were
prepared by grafting thermosensitive poly (N-isopropylacrylamide) (PNIPAM) on the surface of silica (SiO2)-coated Fe3O4 nanoparticles with the particle size of 18.8 ± 1.6 nm. Adsorption and desorption behavior of bovine serum albumin (BSA) on
the surface of PNIPAM-grafted SiO2/Fe3O4 nanoparticles was studied, and the results indicated that these nanoparticles were able to absorb protein at temperature
above the lower critical solution temperature (LCST) and to be desorbed below the LCST. Cytotoxicity studies conducted on
Chinese hamster ovary (CHO-K1) cells using methyl tetrazolium (MTT) assays revealed that cell viability of 1 mg/mL PNIPAM-grafted
nanoparticles was slightly decreased after 24 h of incubation as compared to the lower concentration of nanoparticles. Furthermore,
the concentration of 0.5 mg/mL PNIPAM-grafted nanoparticles was totally biocompatible for 48 h, but had low cytotoxicity after
72 h of incubation. These PNIPAM-grafted nanoparticles did not induce morphological change in their cellularity after exposure
for 24 and 108 h. These results demonstrate that PNIPAM-grafted nanoparticles are biocompatible and have potential use as
drug carriers. |
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