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Observation of baker’s yeast strains used in biotransformation by atomic force microscopy |
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| Authors: | Ricardo de Souza Pereira Nivaldo AntÔnio Parizotto Vitor Baranauskas |
| Institution: | (1) Institute de Quimica, Biological Chemistry Laboratory, Universidade Estadual de Campinas, CP.6154, Campinas CEP, 13084-970 SP, Brazil;(2) Biological Science and Health Center, Physiotherapy Department, Universidade Federal de Sao Carlos, Brazil;(3) Semiconductors, Instruments and Photonics Department, Electrical Engineering Faculty, Universidade Estadual de Campinas, Campinas, SP, Brazil |
| Abstract: | Different strains of baker’s yeast(Saccharomyces cerevisiae) were imaged with an atomic force microscope (AFM). The images of uncoated and nonfixed samples were reproducible with high-constrast and nanometer-resolution. Molecules from the polysaccharide surface of the cell wall were pictured and the distance of atoms was measured. The preparation of samples was easy, suggesting that AFM is a useful tool in this type of analyses. |
| Keywords: | Biotransformation Saccharomyces cerevisiae atomic force microscope baker’ s yeast bud scar |
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