High-performance liquid chromatographic determination/identification of oxytetracycline and sulphadimidine in meat and eggs |
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Authors: | N. Furusawa |
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Affiliation: | (1) Faculty of Human Life Science, Osaka City University, Sumiyoshi-ku, 558-8585 Osaka, Japan |
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Abstract: | Summary A rapid method for the simultaneous determination/identification of residual oxytetracycline (OTC) and sulphadimidine (SDD) in meats (beef, pork, chicken) and eggs by high-performance liquid chromatography (HPLC) was developed. The extraction of OTC and SDD was performed using a Sep-Pak? CN cartridge. The extracts contained OTC/SDD analytes when examined by HPLC using a LiChrospher? 100 RP-8 end-capped column and a mobile phase of acetonitrile-acetic acid-water (28:4:68, v/v/v) with a photodiode array detector. The average recoveries from spiked samples (0.1 μg g−1 and 1.0 μg g−1) were in excess of 80.2% with coefficients of variation between 1.5 and 5.0%. The limits of detection for OTC and SDD were 0.05 and 0.02 μg g−1, respectively. |
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Keywords: | Column liquid chromatography Sep-Pak CN cartridge Oxytetracycline Sulphadimidine Meat and eggs |
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