Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation |
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Authors: | Leite Rodrigo Simões Ribeiro Bocchini Daniela Alonso Martins Eduardo Da Silva Silva Dêenis Gomes Eleni Da Silva Roberto |
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Institution: | 1.Laboratório De Bioquímica E Microbiologia Aplicada, IBILCE—Instituto De Biociências, Letras E Ciências Exatas, UNESP—Universidade Estadual Paulista, Rua Cristóv?o, Colombo, 2265, S?o José Do Rio Preto, 15054-000, S?o Paulo, CEP, Brazil ; |
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Abstract: | This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL beta-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and beta-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75 degrees C, respectively. These enzymes remained stable between a wide range of pH. The beta-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75 degrees C for 1 h. |
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