Application of pressure cycling technology to tissue sample preparation for 2-DE |
| |
Authors: | Ringham Heather Bell Richard L Smejkal Gary B Behnke James Witzmann Frank A |
| |
Institution: | Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202-2111, USA. |
| |
Abstract: | 2-DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post-translational modification. One of 2-DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology. |
| |
Keywords: | 2‐DE Pressure cycling Sample preparation Solubilization |
本文献已被 PubMed 等数据库收录! |
|