Electrode potential-controlled DNA damage in the presence of copper ions and their complexes. |
| |
| Authors: | Miroslav Fojta Lud?k Havran Tatiana Kubicárová Emil Palecek |
| |
| Institution: | Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno, Czech Republic. fojta@ibp.cz |
| |
| Abstract: | Supercoiled (sc) DNA immobilized at the surface of a hanging mercury drop electrode was cleaved by reactive oxygen species generated by an electrochemically modulated reaction of copper ions, hydrogen peroxide and/or oxygen. The cleavage was observed in a certain potential region where redox cycling of DNA-bound Cu(II)/Cu(I) took place. In the presence of 1,10-phenanthroline the maximum efficiency of DNA cleavage was shifted to more negative potentials and the effect was enhanced. |
| |
| Keywords: | |
|
|
