Detecting phase transitions in phosphatidylcholine vesicles by Raman microscopy and self-modeling curve resolution |
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Authors: | Fox Christopher B Uibel Rory H Harris Joel M |
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Institution: | Department of Bioengineering, University of Utah, 50 South Central Campus Drive, Salt Lake City, Utah 84112-9202, USA. |
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Abstract: | The study of phospholipid phase transitions is important for understanding drug- and protein-membrane interactions as well as other phenomena such as trans-membrane diffusion and vesicle fusion. A temperature-controlled stage on a confocal Raman microscope has allowed phase transitions in optically trapped phospholipid vesicles to be monitored. Raman spectra were acquired and analyzed using self-modeling curve resolution, a multivariate statistical analysis technique. This method revealed the subtle spectral changes indicative of sub- and pretransitions and main transitions in vesicles composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The Raman scattering results were compared to differential scanning calorimetry (DSC) experiments and found to be in good agreement. This method of observing lipid phase transition profiles requires little sample preparation and a minimal amount of lipid (
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