Synthesis of NBD-alpha-galactosylceramide and its immunologic properties |
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Authors: | Sakai T Ehara H Koezuka Y |
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Institution: | Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan. |
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Abstract: | formula: see text] A representative alpha-galactosylceramide (alpha-GalCer), KRN7000, can activate NKT cells through CD1d molecules, which play an essential role in the generation of the strong antitumor activity of KRN7000. Our previous study has demonstrated that alpha-GalCer binds directly to CD1d molecules. However, it is controversial whether CD1d binds alpha-GalCer in endosomal compartments. To address this question, we synthesized NBD-alpha-GalCer, which has strong fluorescent properties. We found that the NBD-alpha-GalCer has immunostimulatory activity that is stronger than that of KRN7000. |
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