Study of human neutrophil peptides in saliva by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry |
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| Authors: | Ming‐Hui Yang Li‐Hua Lo Yi‐Hsuan Chen Jentaie Shiea Pei‐Chang Wu Yu‐Chang Tyan Yuh‐Jyh Jong |
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| Affiliation: | 1. Department of Chemistry, National Sun Yat‐Sen University, Taiwan;2. National Sun Yat‐Sen University‐Kaohsiung Medical University Joint Research Center, Taiwan;3. Department of Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan;4. Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Taiwan;5. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan |
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| Abstract: | Matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry is used to rapidly characterize the human neutrophil peptides – HNP 1, 2, and 3 – in saliva. The saliva excreted from the parotid and sublingual/submandibular glands of 70 individuals were collected and examined using MALDI‐TOF. The MALDI approach requires no sample pretreatment other than mixing the saliva‐absorbing material with the matrix and drying under ambient conditions. Tissue paper was the best material for collecting the saliva samples because of its strong texture and high absorbance, and sinapinic acid was the best MALDI matrix for the analysis of the HNPs. HNPs were detected in almost all the samples collected from the parotid glands, with no obvious differences among age or gender. In contrast, the distribution of the HNPs in the samples collected from the sublingual/submandibular glands was age‐dependent: no HNPs were detected for those collected from individuals younger than 30, but the HNPs were present in all of the samples collected from those older than 60 years. The increased probability of detecting saliva HNPs with age suggests that HNPs may function as a biomarker for aging. Copyright © 2009 John Wiley & Sons, Ltd. |
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