Optimization of the ion-pair high-performance liquid chromatographic separation of purine derivatives in erythrocytes, thymocytes and liver mitochondria |
| |
| Authors: | R T Toguzov Y V Tikhonov A M Pimenov Dubiel" target="_blank">W Prokudin VYuDubiel M Ziegler G Gerber |
| |
| Institution: | Department of Biochemistry, Central Research Laboratory, N.I. Pirogov 2nd Moscow Medical Institute, U.S.S.R. |
| |
| Abstract: | Various methods are described for the analysis of purine derivatives in biological samples by ion-pair high-performance liquid chromatography (HPLC) with both gradient and isocratic systems. A new approach is proposed that is suitable for the separation of nuclei acid constituents in different cells with a specific enzymatic activity pattern. The ion-pair HPLC methods were developed for the analysis of erythrocytes, lymphocytes and mitochondria acid-soluble fractions in clinical and experimental studies of normal and altered nucleotide metabolism. The results of studies of purine metabolite redistribution in mouse liver mitochondria during a 30-min incubation at 37 degrees C and data on purine metabolic alterations in mouse thymocytes during hepatoma growth are discussed. |
| |
| Keywords: | |
|
|
