Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation |
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Authors: | Montserrat Sarrà Josep A Pérez-Pons Francesc Gòdia Carlos Casas Alvero |
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Institution: | (1) Departament d’Enginyeria Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08 193 Bellaterra, Barcelona, Spain;(2) Institut de Biologia Fonamental and Departament de Bioquímica i Biologia Molecular, Universitat Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain |
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Abstract: | A fermentation strategy, based on the controlled feeding of growthlimiting nutrients in order to maintain metabolic activity
for extended periods, has been examined in the case of the production of a hybrid antibiotic by a transformed strain ofStreptomyces lividans TK21. The fed-batch operation did not improve the results obtained with batch operation. Continuous cultures on defined medium
showed stable levels of biomass concentration, but antibiotic production ceased when continuous operation was started. The
results obtained indicate the critical influence that morphology of the cell aggregates has on metabolic activity. The antibiotic
is produced only in culture conditions providing growth in compact mycelial pellets. |
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Keywords: | Hybrid antibiotic Streptomyces lividans fed-batch continuous cultivation |
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