| 8-羟基脱氧鸟苷在壳聚糖/石墨烯修饰电极上的电化学及DNA氧化损伤检测 |
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| 引用本文： | 安玲玲,路毛凤,邓玲玲,杜江燕. 8-羟基脱氧鸟苷在壳聚糖/石墨烯修饰电极上的电化学及DNA氧化损伤检测[J]. 应用化学, 2014, 31(2): 200-205. DOI: 10.3724/SP.J.1095.2014.30179 |
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| 作者姓名： | 安玲玲 路毛凤 邓玲玲 杜江燕 |
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| 作者单位： | (1.南京师范大学化学与材料科学学院；2.江苏省生物功能材料重点实验室；;3.江苏省新型动力电池重点实验室 南京 210046) |
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| 基金项目： | 江苏高校优势学科建设工程资助项目（164320H106） |
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| 摘 要： | 采用循环伏安(CV)、线性扫描伏安(LSV)和示差脉冲伏安(DPV)等方法研究了8-羟基脱氧鸟苷(8-OHdG)在壳聚糖(Chi)/石墨烯(GR)修饰的玻碳电极(GCE)上的电化学行为，8-OHdG在该修饰电极上氧化峰电流与其浓度在3.5×10-7~1.4×10-4 mol/L范围内呈良好的线性关系，检测限为6.4×10-8 mol/L(S/N=3)。 将Chi/GR/GCE用于检测DNA氧化损伤，8-OHdG在修饰电极上的氧化峰电流与损伤的DNA质量浓度在10~300 mg/L范围内呈良好的线性关系，损伤DNA检出限为0.026 mg/L(S/N=3)。
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| 关 键 词： | 羟基脱氧鸟苷 壳聚糖 石墨烯 DNA氧化损伤 电化学检测 |
| 收稿时间： | 2013-04-12 |
| 修稿时间： | 2013-06-16 |
Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode andDetection of DNA Oxidative Damage |
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| AN Lingling,LU Maofeng,DENG Lingling,DU Jiangyan. Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode andDetection of DNA Oxidative Damage[J]. Chinese Journal of Applied Chemistry, 2014, 31(2): 200-205. DOI: 10.3724/SP.J.1095.2014.30179 |
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| Authors: | AN Lingling LU Maofeng DENG Lingling DU Jiangyan |
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| Affiliation: | (1.College of Chemistry and Materials Science,Nanjing Normal University;2.Jiangsu Key Laboratory of Bio-functional Material;3.Jiangsu Key Laboratory of New Power Batteries,Nanjing 210046,China) |
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| Abstract: | The electrochemical behavior of 8-hydroxydeoxyguanosine(8-OHdG) at the chitosan(Chi)/graphene(GR) modified glassy carbon electrode(GCE) was investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. A linear relationship between the oxidative peak current and concentrations of 8-OHdG at the modified electrode was established in the range of 3.5×10-7~1.4×10-4 mol/L with a detection limit of 6.4×10-8 mol/L(S/N=3). Chi/GR/GCE was used for the detection of DNA oxidative damage, it showed that the oxidative peak current of 8-OHdG at the modified electrode was linear with concentration of damaged DNA in the range of 10~300 mg/L with a detection limit of 0.026 mg/L(S/N=3). |
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| Keywords: | hydroxydeoxyguanosine chitosan graphene DNA oxidative damage electrochemical detection |
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