Microsomal electrodes for reduced nicotinamide adenine dinucleotide and its phosphate,glucose-6-phosphate and ascorbate |
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| Authors: | F. Schubert F. Scheller D. Kirstein |
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| Affiliation: | Central Institute of Molecular Biology, Academy of Sciences of the GDR, Department of Applied Enzymology, 1115 Berlin-BuchE. Germany |
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| Abstract: | Rat liver microsomes have been immobilized in a membrane by gelatin entrapment. The resulting membranes can be attached to an oxygen electrode to provide a sensor for several compounds. NADPH and NADH are determined by utilizing the liver microsomal NADPH oxidase activity, which is, at least in part, due to the cytochrome P-450 system. The calibration graph for NADPH is linear up to 1 mM. A multi-enzyme system localized in liver microsomes allows the determination of 20–800 μM glucose-6-phosphate. The non-enzymatic lipid peroxidation in liver microsomes, which is induced by ascorbate, allows 0.5–2.5 mM ascorbate to be determined. |
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