A label-free electrochemical immunoassay for IgG detection based on the electron transfer |
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Authors: | Qiu Li-Ping Wang Cun-Chang Hu Peng Wu Zai-Sheng Shen Guo-Li Yu Ru-Qin |
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Institution: | a State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China b Department of Chemistry, Xiangnan University, Chenzhou 423000, PR China |
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Abstract: | In this study, a highly selective, label-free electrochemical immunoassay strategy based on the charge transport through the multilayer films associated with the electrocatalytic reduction of Fe(CN)6]3− is proposed using human immunoglobulin G (human IgG) as the model analyte. The antibody-antigen complex formed on the sensing interface can efficiently induce change of the surface charge characteristics, the conductivity of multilayer film and/or electron transfer distance, resulting in an immunoreaction signal. The current reduction is proportional to the amount of analyte. Under the optimized experimental conditions, the proposed sensing strategy provides a linear dynamic range from 10 to 104 ng mL−1 and a detection limit of 3 ng mL−1, indicating an improved analytical performance. This possibly makes it a potential alternative in bioanalysis of proteins and other molecules. |
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Keywords: | Label-free Electrochemistry Immunoassay Electron transfer Methylene blue Gold nanoparticles |
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