Amplified electrochemical DNA sensor using peroxidase-like DNAzyme |
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| Authors: | Guo Qiuping Bao Yanyan Yang Xiaohai Wang Kemin Wang Qing Tan Yuyu |
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| Institution: | State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, PR China |
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| Abstract: | A novel electrochemical DNA sensor was developed here by using peroxidase-like G-quadruplex-based DNAzyme as a biocatalytic label. A hairpin structure including the G-quadruplex-based DNAzyme in a caged configuration and the target DNA probe were immobilized on Au-electrode surface. Upon hybridization with the target, the hairpin structure was opened, and the G-quadruplex-based DNAzyme was generated on the electrode surface, triggering the electrochemical oxidization of hydroquinone by H2O2, which provide a quantitative measure for the detection of the target DNA. The DNA target was analyzed with a detection limit of 0.6 nM. This method is simple and easy to design without direct conjugation of redox-active element. |
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| Keywords: | G-quadruplex-based DNAzyme Electrochemical DNA sensor Amplification Nucleic acids |
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