Nanostructured platform for the detection of Neisseria gonorrhoeae using electrochemical impedance spectroscopy and differential pulse voltammetry |
| |
Authors: | Renu Singh Zimple Matharu Avanish Kumar Srivastava Seema Sood Rajinder Kumar Gupta Bansi Dhar Malhotra |
| |
Institution: | 1. Department of Science & Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory (Council of Scientific & Industrial Research), Dr. K.S. Krishnan Marg, New Delhi, 110012, India 3. School of Biotechnology, Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi, 110006, India 2. Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 110002, India 4. Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Main Bawana Road, Delhi, 110042, India
|
| |
Abstract: | We report on a nanocomposite based genosensor for the detection of Neisseria gonorrhoeae, a bacterium causing the sexually transmitted disease gonorrhoea. Amino-labeled probe DNA was covalently immobilized on electrochemically prepared polyaniline and iron oxide (PANI-Fe3O4) nanocomposite film on an indium tin oxide (ITO) electrode. Scanning electron microscopy, transmission electron microscopy, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) techniques have been employed to characterize surface of the modified electrode. The genosensor has detection limits of 1?×?10-15 M and 1?×?10-17 M, respectively, using the EIS and DPV techniques. This biosensor can discriminate a complementary sequence from a single-base mismatch and from non-complementary DNA, and has been utilized for detection of DNA extracted from N. gonorrhoeae culture, and from patient samples with N. gonorrhoeae. It is found to exhibit good specificity for N. gonorrhoeae species and shows no response towards non-gonorrhoeae type of Neisseria species (NgNs) and other gram-negative bacterias (GNBs). The affinity constant for hybridization calculated using the Langmuir adsorption isotherm model is found to be 3.39?×?108 M-1. Figure Electrochemically deposited nanostructured platform for Neisseria gonorrhoeae detection. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|