Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
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| Authors: | Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Soon Guan Tan Fatimah Abu Bakar |
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| Institution: | (1) Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, 88400, Malaysia;(2) Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia;(3) Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia;(4) Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia;(5) Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia |
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| Abstract: | Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium
trifluoromethanesulfonate (EMIM]Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold
electrode (AuE). The electrochemical properties of the EMIM]Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied.
Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of
1.0 × 10−18 to 1.82 × 10−4 mol L−1, and with the detection limit of 1.0 × 10−19 mol L−1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification
of Trichoderma harzianum. |
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