辐射诱导促进AdCMV-p53 转染前列腺癌细胞 |
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引用本文: | 张红,刘兵,高清祥,谢漪,郝冀方,段昕,周清明,王燕玲.辐射诱导促进AdCMV-p53 转染前列腺癌细胞[J].原子核物理评论,2007,24(2):112-116. |
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作者姓名: | 张红 刘兵 高清祥 谢漪 郝冀方 段昕 周清明 王燕玲 |
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作者单位: | 1 中国科学院近代物理研究所, 甘肃 兰州 730000; 2 兰州军区疾病控制中心, 甘肃 兰州 730020; 3 兰州大学生命科学院, 甘肃 兰州 730000 |
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基金项目: | 国家自然科学基金;甘肃省科技攻关项目;甘肃省自然科学基金 |
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摘 要: | 用腺病毒重组体(AdCMV p53/GFP)转染经0.5, 1.0和2.0 Gy γ射线辐射处理的前列腺癌细胞PC 3( nullp53)], 用克隆形成法检测细胞增殖能力, 用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。 结果提示, 辐射诱导使腺病毒重组体转染PC 3细胞提高7%—39%。 辐射联合 AdCMV p53 转染组p53表达水平提高18.5%—35.4%。 与单纯 AdCMV p53 转染组和单纯辐射组相比, 辐射联合 AdCMV-p53 转染组细胞存活率分别降低25%—64%和22%—65%。 To determine whether low dose pre irradiation could enhance adenovirus mediated p53 transfer and expression in human prostate adenocarcinoma, the PC 3 cells were pre exposed to γ rays, and then infected with replication deficient adenovirus recombinant vectors, containing human wild type p53 (AdCMV p53) or green fluorescent protein gene (AdCMV GFP) respectively (γ ray irradiation + AdCMV p53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMV GFP infection groups were 7%—39% more than those in AdCMV GFP infection groups. The p53 levels in the γ irradiation + AdCMV p53 infection groups were 18.5%—35.4% more than those in AdCMV p53 infection groups (p<0.05),suggesting that low dose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC 3 cells. The survival fractions for the γ irradiation + AdCMV p53 infection groups were 25%—64%, 22%—65% less than those for AdCMV p53 infection, or γ irradiation groups, respectively (p<0.05).
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关 键 词: | p53基因 辐射 腺病毒载体 前列腺癌 辐射敏感性 |
文章编号: | 1007-4627(2007)02-0112-05 |
收稿时间: | 1900-01-01 |
修稿时间: | 2007-02-14 |
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