Simplex optimization of acoustic assay for plasminogen activators |
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Authors: | Mirnader Ghazali Gordon L Hayward |
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Institution: | (1) Present address: School of Engineering, University of Guelph, Guelph, N1G 2W1, Canada;(2) Faculty of Engineering, University of Isfahan, Isfahan, 81746–73441, Iran |
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Abstract: | This article discusses the optimization of a newly developed method for measuring the activity of plasminogen activators using
a thickness-shear-mode acoustic sensor. A variable-size simplex algorithm was used for optimization. Preliminary tests were
performed to design the first simplex. A desirability function was defined to translate each performance value to a membership
value of 0 to 1. If there was more than one performance variable, their membership values were translated to an aggregated
membership value using another function that considers their individual influence on sensor performance. Two rounds of optimization
were carried out for streptokinase followed by a single optimization for tissue-type plasminogen activator. In the last optimization,
ratios of control variables were used in order to reduce the number of parameters and to formulate easily adjustable assay
conditions. The results showed the usefulness of the simplex method for optimizing this type of assay, and the importance
of preliminary tests and prior knowledge in providing rapid convergence using fewer experiments. The optimized plasminogen
activator assay can be considered a reference method for measurement of all members of this drug class. |
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Keywords: | Optimization Simplex Plasminogen activator Thickness-shear mode Acoustic sensor |
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