Biotransformation of aromatic ketones and ketoesters with the non-conventional yeast Pichia glucozyma |
| |
| Institution: | 1. Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA;2. Department of Neuropsychiatry, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, Toyama, Japan;3. National Centre for Register-Based Research, Aarhus University, Aarhus, Denmark;4. Neuropsychiatry Program, Sheppard Enoch Pratt Hospital, Baltimore, MD, USA;5. Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA |
| |
| Abstract: | The non-conventional yeast Pichia glucozyma CBS 5766 has been used for the biotransformation of different aromatic ketones and ketoesters. The growth and biotransformation conditions were optimised for the reduction of acetophenone and under optimised conditions, propiophenone, butyrophenone and valerophenone were reduced to the corresponding (S)-alcohols with high yields and enantioselectivity. Ketoreductase(s) of Pichia glucozyma showed high catalytic activities also towards aromatic β- and γ-ketoesters, being often competitive with esterase(s). These concurrent activities allowed for the preparation of hydroxyesters, hydroxyacids and lactones often in a very selective manner. |
| |
| Keywords: | Aromatic ketones Aromatic ketoesters Reduction Enantioselective Ketoreductase Esterase |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
